Journal: Biomolecules

Article Title: Evidence for FOXL2 Association with the Tsc1 Regulatory Region in Mice

doi: 10.3390/biom16040510

Figure Lengend Snippet: Expression and localization of TSC1/FOXL2 and KITL/VASA1 in P7 mouse ovaries. ( a ) Immunofluorescence was carried out in sections of P7 Foxl2 WT mouse ovary, using anti-FOXL2 and anti-TSC1 antibodies. FOXL2 is expressed in the GCs of primordial and primary follicles but not in the oocytes. TSC1 is expressed both in the GCs and in the oocytes of maturating follicles. The yellow box highlights the zoomed-in region showing co-localisation at the cell level. ( b ) Immunofluorescence was carried out in sections of P7 Foxl2 WT and Foxl2 −/− mouse ovaries, using anti-KITL and anti-VASA1 antibodies. In blue, nuclear counterstaining performed with DAPI. Immunofluorescence imaging was acquired using a Leica DMIRE2-TCS-SL Confocal Laser Scanning microscope and with a HiRes CCD camera and processed on a Deltavision system version 5.10. Magnification bars indicate 50 μm at 20× and 63×, 10 μm at 100×.

Article Snippet: The antibodies used were anti-FOXL2 (#ab5096, 1:25, Abcam, Cambridge, UK), anti-TSC1 (#MBS176028, 1:200, MyBioSource, San Diego, CA, USA), anti-KITL (#bs-0545R, 1:200, Bioss, Woburn, MA, USA), and anti-VASA ((#A21206, BD Pharmingen 560189, 1:100).

Techniques: Expressing, Immunofluorescence, Imaging, Laser-Scanning Microscopy